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(A) Difference absorption spectra from UV/Vis reconstitution from 1 (red) – 60 (blue) minutes. (B – C) Kinetic profile of UV-Vis absorbance at (B) 620 nm and (C) 390 nm in protiated (black) and deuterated (green) buffers. Relative EPR intensities of intermediates at (B) 30 K and (C) 6.7 K are overlaid (filled squares).
