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Gating strategy for identification of antigen‐experienced B cells in unimmunized mice. B220+ cells in the spleen were sequentially gated as IgD– CD21− CD93− CD23+ to identify total antigen‐experienced cells and then as IgM+ and IgM– for identification of the unswitched and switched components, respectively (a). Gating for identification of CD73+, CD80+ and CD273+ cells on IgM+ (b) and IgM– (c) antigen‐experienced cells.
