Figure 2.

IgM⁺ antigen‐experienced cells share features of memory. Expression of transcripts (as labelLed) in sorted IgM⁺ and IgM^– antigen‐experienced cells from unimmunized mice relative to 24‐hr lipopolysaccharide (LPS) blasts (a). Immunoglobulin in supernatants of sorted cells stimulated with 10 μg/ml LPS in the absence (without) or presence (with) of 1 μg/ml aphidicolin for 84 hr. Activated B cells are spleen cells pre‐stimulated with LPS for 72 hr and re‐stimulated with or without aphidicolin for 48 hr (b). (ASCs)/10⁵ cells that were stimulated with LPS ± aphidicolin for 72 hr. Activated B cells are spleen cells pre‐stimulated with LPS for 72 hr and re‐stimulated with or without aphidicolin for 48 hr (c). Data are shown as mean ± SD of replicates (a), mean ± SEM of triplicate cultures (b), and mean ± SEM of triplicate cultures, with cells from each culture loaded onto six wells each for ASC assay (c).