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. 2017 Aug 23;152(4):638–647. doi: 10.1111/imm.12805

Figure 1.

Figure 1

IL‐27P28‐deficient mice had a reduced Sca‐1+ memory pool of T cells. Splenocytes from naive wild‐type (WT) B6 and P28−/− mice were analysed by flow cytometry. Spleen CD4+ and CD8+ T cells and their subsets (a), based on the expression of CD62L and CD44, were analysed for the expression of Sca‐1 (b). Sca‐1 expression in CD4+ and CD8+ T cells and their subsets were quantified (c). Three to five mice were used in each group for this experiment. Data are expressed as means of individual determinations ± SE and represent three experiments using both male and female mice. Statistical analysis was performed using the unpaired Student's t‐test. *P < 0·05; **P < 0·01; ***P < 0·001. N.S., not significant.