Figure 4.

Adeno‐associated virus (AAV) vector‐delivered interleukin‐27 (IL‐27) induces Sca‐1 expression in T cells. (a) Wild‐type (WT) mice were injected with AAV‐ctrl, AAV‐IL‐27 or AAV‐IL‐30 viral vectors intramuscularly at a dose of 2 × 10¹¹ DNAse resistant particle (DRP)/mouse, and serum IL‐27 or IL‐30 levels were measured by ELISA 2 weeks later. Spleen CD4⁺ and CD8⁺ T cells and their subsets were analysed for the expression of Sca‐1 (b). Sca‐1 expression in CD4⁺ (c) and CD8⁺ (d) subsets was quantified. Three mice were used in each group for this experiment. Data are expressed as means of individual determinations ± SE and represent three independent experiments using both male and female mice. Statistical analysis was performed using the unpaired Student's t‐test. #P < 1 × 10⁻⁵.