Figure 6.

Expression of transcription factors and stemness genes in T cells from Adeno‐associated virus–interleukin‐27 (AAV‐IL‐27) and AAV‐ctrl‐treated mice. C57BL/6 mice were treated with AAV‐ctrl or AAV‐IL‐27 viral vectors intramuscularly at a dose of 2 × 10¹¹ DNAse resistant particle (DRP)/mouse. Two weeks later CD4⁺ and CD8⁺ T cells were sorted from the spleens by FACS. Total RNA was prepared from purified T cells and quantitative PCR was used to quantify transcription factors associated with T‐cell differentiation and stemness in CD4⁺ (a) and CD8⁺ T cells (b). Samples were pooled from three mice in each group. Data are expressed as means of individual determinations ± SE of three replicates, and represent two independent experiments. Statistical analysis was performed using the unpaired Student's t‐test. *P < 0·05; **P < 0·01. nd, not detectable.