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Effect of an anti‐human neurofilament light (NF‐L) antibody on neuronal viability. Differentiated PC12 cells were treated for 24, 48 and 72 hr with 20 μg/ml rabbit anti‐human NF‐L antibody or rabbit IgG as a control of the assay. Cells were incubated with MTT (0·5 mg/ml) for the last 4 hr of incubation, then supernatants were discarded and 200 μl DMSO was added to solubilize the formazan crystals. Absorbance was determined at 590 nm using an ELISA reader. ***P < 0·001, **P < 0·01.
