Figure 4.

Immunohistological staining and quantification of glial fibrillary acidic protein (GFAP) 24 h after dexamethasone (DX) administration. (a) Representative immunofluorescence of 30 μm coronal sections stained with anti‐GFAP antibodies (red) and 4',6‐diamidino‐2‐phenylindole (DAPI) (blue nuclei) in two cornu ammonis (CA1 and CA2) regions and the cortex (R1 and R2). The pictures derive from naive, lipopolysaccharide (LPS)‐treated mice without additional treatment, and animals treated with saline or DX. (b) Table shows the mean ± standard deviation of the percentage of pixels/µm². The effects of the different treatment in GFAP expression in each region were compared. Data labelled with the same letter are not significantly different from each other, whereas those with different letters are significantly different. [Colour figure can be viewed at wileyonlinelibrary.com]