Figure 3.

Lamina propria dendritic cells (LP‐DCs) are impaired at acquiring epithelial and luminal antigens in the absence of CCR6. (a) Photomicrographs of cytospins of CD103⁺ LP‐DCs from wild‐type (WT) and CCR6^−/− mice stained with cytokeratin 18 (cyt18; red) and DAPI (blue). (b) Quantification of the percentage of CD103⁺ and CD103⁻ LP‐DCs from WT and CCR6^−/− mice staining for cytokeratin 18. (c) Flow cytometry plots and (d) graphical representation of small intestine lamina propria mononuclear phagocytes (LP‐MNPs) after gavage of fluorescent bovine serum albumin (BSA‐647) demonstrating CD103⁻ LP‐MNPs from CCR6^−/− mice are impaired at acquiring gavaged labelled protein. (e) Quantification of BSA‐647⁺ LP‐MNPs following in vitro culture with various concentrations of BSA‐647 demonstrates no significant difference in the ability of CCR6‐deficient LP‐MNPs to acquire BSA‐647 in culture. n = 3 in each panel. *P < 0·05, ns = not significant. Scale bar in (a) = 20 μm. [Colour figure can be viewed at wileyonlinelibrary.com]