Figure 7.

Immune responses in the mesenteric lymph node to luminal, but not systemic antigen, are impaired in the absence of CCR6. (a) Flow cytometry histograms of lamina propria (LP) ‐MNPs from wild‐type (WT) and CCR6‐deficient mice demonstrating similar levels of in CCR7 expression in the absence of CCR6. (b) Absolute numbers of mesenteric lymph node (MLN) dendritic cells (DCs) in WT and CCR6‐deficient mice. (c) Representative flow cytometry plots and (d) graphical representation of the resident and migratory MLN DC populations from WT and CCR6‐deficient mice. (e) Flow cytometry plots and histograms of adoptively transferred ovalbumin (OVA) ‐specific CD4⁺ OTII T cells from the MLNs of WT or CCR6‐deficient mice given that (e) luminal or (f) intravenous OVA demonstrates that MLN T‐cell responses to luminal but not systemic antigen are impaired in the absence of CCR6. Panels a, c, e, and f are representative of three replicates, n = 3 panels b and d. ns = not significant, *P < 0·05. [Colour figure can be viewed at wileyonlinelibrary.com]