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. 2017 Aug 11;152(4):562–573. doi: 10.1111/imm.12792

Figure 6.

Figure 6

Down‐regulation of Epstein–Barr virus (EBV) encoded genes in Farage decreased specific chemotaxis induced by cytokines. (a) Immunoblot analysis of total cell extracts of dominant negative EBV nuclear antigen 1 (dnEBNA1) ‐Farage cultured with or without 1 μg/ml doxycycline (Dox) for 20 days with β‐actin and EBNA1 antibodies. (b) The expression of CXCR4, and CCR7 on dnEBNA1‐Farage cultured with or without 1 μg/ml Dox for 20 days and then treated with IL‐4 or IL‐21 for 24 hr was assessed by FACS analysis. (c) dnEBNA1‐Farage cultured with or without 1 μg/ml Dox for 20 days and then treated with IL‐4 or IL‐21 for 24 hr and the chemotactic response induced by CXCL12 and CCL21 in cytokine‐treated and control cells were compared in a 4‐hr Transwell migration assay. The migrated cells were counted by FACS for 60 seconds from the wells that contained cells that migrated to the ligand compared with the input population after subtraction of background migration. The result is presented as relative fold change where migration in non‐treated (NT) cells was arbitrarily defined as 1. The mean values are shown, calculated from three experiments. The error bars indicate ± SD (n = 3).