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. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: J Phys Chem B. 2017 Oct 31;121(44):10228–10241. doi: 10.1021/acs.jpcb.7b08045

Figure 7. Dose-response in fluorescence anisotropy acquired with SELENOK U92C and SELENOS U188S, two short single α-helical transmembrane proteins solubilized in DDM.

Figure 7

(A) Cartoon presenting the transmembrane topography of the SELENOK U92C and SELENOS U188S proteins; (B) Domain organization and the position of relevant Cys and Sec residues of SELENOS and SELENOK. TM stands for the transmembrane region of these proteins; (C) The protein concentration in the well was 200 nM. The initial DDM concentration was 1.3 mM. The FP measurements were carried out using a solution that contained 200 mM NaCl, 50 mM HEPES, pH 7.4 at a temperature of 24°C. Vertical bars represent the magnitudes of the CMC and Kd. All the other experimental conditions were the same as in Fig. 3.