Skip to main content
. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: J Phys Chem B. 2017 Oct 31;121(44):10228–10241. doi: 10.1021/acs.jpcb.7b08045

Table 3. The recorded minima and maxima of the anisotropy with neutral and zwitterionic detergents and β-barrel proteinsa.

This table also illustrates the rotational diffusion coefficients as well as alterations in hydrodynamic radii of the proteomicelles during the detergent desolvation transitions.

DMb rmin c rmax c Dr slow (107 s−1)d Dr fast (107 s−1)d Rh max (nm)e ΔRh (nm)f
OmpG 0.214 ± 0.005 0.327 ± 0.001 0.89 ± 0.02 3.5 ± 0.2 2.6 0.95 ± 0.04
FhuA ΔC/Δ5L 0.219 ± 0.005 0.360 ± 0.001 0.44 ± 0.01 3.3 ± 0.2 3.3 1.6 ± 0.1
FhuA ΔC/Δ5L_25N 0.166 ± 0.003 0.343 ± 0.002 0.66 ± 0.03 5.6 ± 0.2 3.0 1.5 ± 0.1
FhuA ΔC/Δ7L_30N 0.168 ± 0.007 0.312 ± 0.001 5.5 ± 0.4 1.0 ± 0.1 2.4 1.0 ± 0.1
CYMAL-4b rmin c rmax c Dr slow (107 s−1)d Dr fast (107 s−1)d Rh max (nm)e ΔRh (nm)f
OmpG 0.163 ± 0.001 0.326 ± 0.001 0.90 ± 0.01 5.8 ± 0.1 2.6 1.2 ± 0.1
FhuA ΔC/Δ5L 0.242 ± 0.001 0.367 ± 0.001 0.36 ± 0.01 2.6 ± 0.1 3.6 1.7 ± 0.1
FhuA ΔC/Δ5L_25N 0.166 ± 0.001 0.341 ± 0.001 0.69 ± 0.01 5.6 ± 0.1 2.9 1.4 ± 0.1
FhuA ΔC/Δ7L_30N 0.168 ± 0.025 0.345 ± 0.004 0.63 ± 0.05 5.5 ± 1.2 2.9 1.5 ± 0.2
OGb rminc rmaxc Drslow (107 s−1)d Drfast (107 s−1)d Rh max (nm)e ΔRh (nm)f
OmpG 0.153 ± 0.002 0.306 ± 0.001 1.2 ± 0.1 6.4 ± 0.1 2.4 1.0 ± 0.1
FhuA ΔC/Δ5L 0.162 ± 0.002 0.291 ± 0.002 1.5 ± 0.1 5.8 ± 0.1 2.2 0.80 ± 0.02
FhuA ΔC/Δ5L_25N ~0.15 ~0.17 ~5.2 ~6.6 NDh NDh
FhuA ΔC/Δ7L_30N ~0.15 ~0.17 ~5.2 ~6.6 NDh NDh
CHAPSb rmin c rmax c Dr slow (107 s−1)d Dr fast (107 s−1)d Rh max (nm)e ΔRh (nm)f
OmpG NDh 0.318 ± 0.002 1.0 ± 0.1 NDh 2.5 NDh
FhuA ΔC/Δ5L 0.172 ± 0.048 0.292 ± 0.004 0.53 ± 0.2 1.5 ± 0.1 2.2 0.77 ± 0.18
FhuA ΔC/Δ5L_25N ~0.15 ~0.17 ~5.2 ~6.6 NDh NDh
FhuA ΔC/Δ7L_30N ~0.16 ~0.17 ~5.2 ~6.0 NDh NDh
LysoFosb rmin c rmax c Dr slow (107 s−1)d Dr fast (107 s−1)d Rh max (nm)e ΔRh (nm)f
OmpG 0.229 ± 0.006 0.307 ± 0.001 1.2 ± 0.1 3.0 ± 0.2 2.4 0.61 ± 0.04
FhuA ΔC/Δ5L 0.223 ± 0.007 0.330 ± 0.001 0.84 ± 0.01 3.1 ± 0.2 2.7 0.95 ± 0.05
FhuA ΔC/Δ5L_25N 0.177 ± 0.001 0.313 ± 0.001 1.1 ± 0.02 5.0 ± 0.1 2.4 0.96 ± 0.02
FhuA ΔC/Δ7L_30N 0.184 ± 0.003 0.294 ± 0.005 1.4 ± 0.1 4.7 ± 0.1 2.2 0.73 ± 0.06
FhuA ΔC/Δ5L in Gdm-HClg ~0.16 ~0.16 ~6.0 ~6.0 1.4 NAi
a

To reach low detergent concentrations below CMC, the Gdm-HCl-solubilized protein was refolded at various detergent concentrations above the CMC.

b

Full names of the detergents are provided in Experimental and computational methods.

c

rmin was extrapolated for the lowest detergent concentration in the well. rmax was determined at detergent concentrations above the CMC.

d

Dr slow and Dr fast indicate the rotational diffusion coefficients under solvation and desolvation conditions, respectively.

e

Rh max is the maximum hydrodynamic radius of the proteomicelle.

f

ΔRh is the decrease in the hydrodynamic radius, Rh, as a result of the detergent desolvation.

g

Anisotropy was determined in 6 M Gdm-HCl.

h

Not determined.

i

Not applicable.