Figure 3.
miR-92b-3p expression in spinal dorsal horn (L6-S1) samples from rats receiving treatment as described in Fig. 1. Groups include: (A) neonatally-induced cystitis; (B) neonatal cystitis followed by adult re-challenge; (C) adult cystitis. The ordinate value 2−ΔCT corresponds to the miRNA expression relative to endogenous SNORD-95 expression and is represented as mean ± SD, n=5/group, * p<0.05, ** p<0.01 vs saline controls. (D) In situ hybridization demonstrates significant upregulation of intensity of miR-92b-3p expression (** p<0.01 vs saline controls) in zymosan treated rats. The scale bar is 50 µm.