Representative western blots and quantification data for protein expression in
the hypothalamic PVN homogenates of wild-type (WT) and B1R gene deletion (B1RKO)
mice treated with sham or DOCA-salt for three weeks. Protein expression of Nox2,
a catalytic subunit of NADPH Oxidase (A), and inducible nitric
oxide synthase (iNOS, B) were significantly increased in
hypothalamic PVN indicating increased oxidative stress. However, this increase
in oxidative stress was not observed in B1RKO mice. In addition, phosphorylated
and total ASK1 (C), phosphorylated and total JNK (D),
and phosphorylated and total ERK1/2 (E) protein expression in the
PVN indicates activation of ASK1, JNK and ERK1/2 in DOCA-salt hypertension,
which was prevented by B1R gene deletion. n=4/group, PVN tissues from 3
mice were pooled for each sample. Data are presented as mean ±SEM.
Two-way ANOVA, *P<0.05 vs. WT+Sham,
†P<0.05 vs.
WT+DOCA.