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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Pain. 2017 Dec;158(12):2396–2409. doi: 10.1097/j.pain.0000000000001044

Figure 4. Predominantly peptidergic primary afferent neurons innervate the tongue.

Figure 4

Tongue afferent neuron labeling using the lipophilic retrograde tracer, DiI. A) Representative images from co-staining of DiI-positive (red) TG sections with CGRP (top, green), IB4-FITC (middle, green), and NF200 (bottom, green). Co-expression is indicated by yellow overlap (Merge). B) Pooled data from 3 female TG pairs indicated that DiI-positive neurons had predominantly more CGRP-immunolike reactivity (IR) (64.3 ± 13.2%) compared to IB4-IR (13.2 ± 1.6%) and NF200-IR (29 ± 8.9%) populations. C) Histogram of neuronal size distribution of cells retrogradely labeled from the tongue. Tongue afferent neurons are predominately small to medium (soma size 151–450 μm2).