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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Biol Trace Elem Res. 2017 May 9;181(1):54–61. doi: 10.1007/s12011-017-1044-9

Figure 2. DIDS is a specific inhibitor of SELENOP uptake.

Figure 2

(A) HeLa cells were labeled with 75Se selenite or 75Se-SELENOP in the presence of a range of 4,4-Diisothiocyanatostilbene-2,2-Disulfonic Acid (DIDS) as indicated. 75Se labeled proteins were resolved by SDS-PAGE and detected by PhosphorImage analysis. (B) SELENOP was pre-treated with DIDS. Excess DIDS was removed by gel filtration and varying amounts of the DIDS-treated or untreated control protein was used to label endogenous HeLa selenoproteins as above.