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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: Biol Trace Elem Res. 2017 May 9;181(1):54–61. doi: 10.1007/s12011-017-1044-9

Figure 5. Degradation of SELENOP occurs under acidic and reducing conditions.

Figure 5

(A) HepG2 derived conditioned media containing 75Se-SELENOP was incubated at 37 °C for 30 min in varying concentrations of acetic acid and/or 2 mM DTT as indicated. (B) 75Se-SELENOP was purified by nickel affinity chromatography and incubated at 37 °C for 30 min.