Table 1.

Nomenclature used in this manuscript

Term	Description
Endmember	A spectral component of an image. This is often either an exogenously added dye, a fluorescent protein, or tissue autofluorescence.
Target	Target refers to a specific endmember of interest. This could be one fluorescent label, for which a particular study was designed to detect.
Signature, fingerprint	Both of these terms refer to the spectral shape of a given endmember.
Symbol	Variable
k	Image width (pixels)
l	Image height (pixels)
m	Number of endmembers
n	Number of spectral bands
w	Noise
I	Intensity
r⃗	Endmember spectrum
r	Spectral library
x⃗	Measured pixel spectrum
x⃗′	Measured pixel spectrum for modified pixel within a region of interest (contains pixel + target endmember spectrum)
x	Spectral image
a	Abundance
Rcorr	Sample correlation matrix
U	Undesired spectra (spectral library with target endmember spectrum removed)
P	MTMF rejection operator
q⃗	MTMF filter operator