Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Nov 9;7:15136. doi: 10.1038/s41598-017-15427-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Effect of FGF/MAPK inhibition on isolated E3.5 ICMs. (A) Control E3.5 ICM cultured 48 hrs, (B–D) ICMs derived from E3.5 blastocysts and afterwards cultured 48 hr hrs in 2inh: (B) ICM with the outer PE layer, (C) ICM with the outer TE layer, and (D) ICM with outer heterogeneous (PE and TE) layer, (E,F) ICMs derived from E3.5 blastocysts pre-incubated in 2inh. media from the 8-cell stage: (E) ICM with the outer TE layer, (F) Blastocyst with the TE, EPI and PE, (G) The time schedule of inhibitor treatment; red and black arrows indicate the culture periods in the presence or absence of inhibitors, respectively, (H) Composition of epithelial layer in isolated ICMs cultured 48 hrs, (I,J) Polarisation of E3.5 ICMs (isolated from embryos pre-incubated in 2inh. conditions from the 8-cell stage) immediately (I) and 24 hrs after IS (J), (K,L) Localisation of pYAP in E3.5 ICMs (isolated from embryos pre-incubated in 2inh. conditions from the 8-cell stage) immediately (K) and 24 hrs after IS (L), (M,N) Localisation of nuclear YAP in E3.5 ICMs immediately (M) and 24 hrs after IS (N); yellow (*) indicates blastocyst cavity, orange arrows indicate ICM cells devoid of pYAP signal. Scale: 20 μm.