Table 2.
Antibacterial activity of D-Ctl in combination with conventional antimicrobials.
| Pathogens | Combination | MIC alone (µg/mL) | MIC combination (µg/mL) | FIC | FICI | Effect |
|---|---|---|---|---|---|---|
| Escherichia coli MDR | D-Ctl | 8.4 | 4.2 | 0.5 | 1.0 | Additive |
| Cefotaxime | 0.1 | 0.05 | 0.5 | |||
| Fusobacterium nucleatum | D-Ctl | 22 | 11 | 0.5 | 1.0 | Additive |
| Amoxicillin | 0.6 | 0.3 | 0.5 | |||
| Prevotella intermedia | D-Ctl | 10 | 2.5 | 0.25 | 0.5 | Synergistic |
| Amoxicillin | 0.5 | 0.125 | 0.25 | |||
| Parvimonas micra | D-Ctl | 23 | 5.8 | 0.25 | 0.5 | Synergistic |
| Amoxicillin | 0.5 | 0.125 | 0.25 | |||
| Staphylococcus aureus (MSSA) | D-Ctl | 24 | 12 | 0.5 | 0.75 | Additive |
| Methicillin | 1.2 | 0.3 | 0.25 | |||
| Staphylococcus aureus (MRSA) | D-Ctl | 18 | 18 | 1 | 2 | Indifferent |
| Vancomycin | 0.8 | 0.8 | 1 |
The percentage of growth inhibition of the indicated pathogens in the presence of different concentrations of antimicrobials was determined by broth microdilution assays. The MICs of each drug were used to calculate the FIC index of each combination. Each experiment was performed at least in duplicate.