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. 2017 Nov 9;7:15137. doi: 10.1038/s41598-017-15085-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of marginal zinc deficiency on renal parameters at GD18.5. Decreased gene expression of angiotensin converting enzyme (Ace) was associated with a decrease in Ace protein expression within the kidneys of zinc-deficient dams (a). Representative image of Ace expression in a zinc-replete (b) and zinc-deficient (c) kidney section. Ace protein was localised to the proximal convoluted tubules (PCT). Marginal maternal zinc deficiency also decreased glomeruli basement membrane thickness (d). Representative images of a glomerulus in a zinc-replete (e) and zinc-deficient (f) kidney section. Data are median and interquartile range (n = 10 zinc-replete and 9 zinc-deficient dams). Statistical significance was determined using Mann-Whitney Test. *P < 0.05, **P < 0.01. G: glomerulus. T: tubule