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. 2017 Nov 9;8:1380. doi: 10.1038/s41467-017-01704-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Expression and subcellular localization of AIPR-1. a A GFP reporter, expressed under the control of genomic DNA upstream of the aipr-1 initiation site, showed expression in many cell types, such as motor neurons along the ventral nerve cord (VNC), body-wall muscle cells (BWM), amphid sheath cells (Amsh), spermatheca (Sp), and the intestine (Int). b, c AIPR-1 is expressed in all acetylcholine and GABA motor neurons in the ventral nerve cord. Acetylcholine and GABA motor neurons were labeled by expressing mRFP and mStrawberry under the control of Punc-17 and Punc-47, respectively. Images were taken from a segment anterior b and posterior c to the vulva. d, AIPR-1::GFP displayed both diffuse and punctate distribution along the dorsal nerve cord, and the GFP puncta colocalized with the presynaptic marker TagRFP::ELKS-1. Images were taken from a dorsal segment anterior to the vulva. Scale bars, 20 µm a– c and 10 µm d, e