Fig. 7.
Activation of SuMNos1 neurons promotes wakefulness. a NOS1 immunohistochemisty (left, white scale bar 500 µm applies to all panels of a and b) reveals a subpoplulation of SuM neurons that only rarely contain vgat, shown as gray-white neurons on the merged image (right). b NOS1 immunohistochemistry (left) in a vglut-Cre L10 reported mouse (center) showing that NOS1 neurons are a subset of vglut2 neurons, with no examples of neurons with NOS immoreactivity that were not vglut2 positive (right, merge). c Cartoon showing AAVx-FLEX-hM3Dq-mCherry recombining in NOS1 Cre expressing neurons. d Injection site showing DAB reaction product in neurons positive for mCherry, with black nuclear reaction product in cFos positive neurons after CNO injection (black scale bar, bottom right, 500 µm). e, f Hypnograms from two mice after vehicle and CNO, shows a strong effect of NOS1 cellular activation on wakefulness, with g, h the CSA (0−100 Hz, black bar shows the dark period, short white bars represent 20 min and are spaced 1 h apart) from the areas shown in e and f by the blue and red boxes, respectively. i Pooled data from seven mice showing the percentage of wakefulness in 1 h bins after injection (****p < 0.0001, **p = 0.0023, *p = 0.0248)