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. 2017 Nov 9;7:15227. doi: 10.1038/s41598-017-15151-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Polymer nanodiscs. (A) Illustration of a PND with reconstituted MsbA. The amphiphilic block copolymer membrane patch comprised of hydrophobic membrane-forming (gold) and hydrophilic membrane-surface blocks (gray) is encased within two MSPs (green ribbon coils). One reconstituted MsbA dimer (subunits in purple and blue) is also shown. This PND model is derived from our spectroscopy and microscopy analysis, and available information on the LND structure. (B) Chemical structure of the amphiphilic block copolymer HPBD-b-(P4MVP₂₈)₂. The hydrophobic HPBD block (i.e., hydrogenated 1,4-polybutadiene) and the hydrophilic P4MVP blocks (i.e., poly(4-vinyl-N-methylpyridine iodide) are shown in yellow and white, respectively. The letters m and n signify the number of repeating units. See Supplementary Information for details.