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. 2017 Nov 9;7:15227. doi: 10.1038/s41598-017-15151-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stability of PNDs. (A) Typical examples illustrating hydrodynamic diameter distributions determined by DLS of PNDs and LNDs incubated at 37 °C. (B) Aggregation of PNDs and LNDs kept for 1 week at 4 °C, 20 °C or 37 °C, as revealed by DLS. The figure shows the percentage of light scattered (LS) by nanodiscs consisting of a monodisperse population of ~11-nm diameter assessed from size-intensity distributions such as those shown in panel A. See Materials and Methods for details. Data are means ± SEM of 6–7 independent experiments in all conditions, except for PNDs and LNDs at t = 0 (n = 13 and 11, respectively), and PNDs at 20 °C in days 6 and 7, where n = 1 and 2, respectively, and the single measurement and average are reported. SEMs smaller than the symbols are not shown. Differences between PNDs and LNDs were statistically significant (P < 0.001) from day 1 at 37 °C and day 2 onwards at 4 and 20 °C.