Fig. 2.

Ad-TD-LUC and IL-12-armed viruses replicate in and are cytotoxic cancer cell lines. a, b To assess virus cytotoxicity, cell proliferation assays (Promega) were carried out in all human a and Syrian hamster b tumor cell lines. Cells were infected with Ad5, Ad-TD-LUC or Ad-TD-IL-12 or Ad-TD-nsIL-12 and incubated for 6 days at 37 ^oC. The amount of virus particles required to kill 50% of the cells is shown as EC50 (PFU/cell) and shown as mean and SEM compared using an independent t-test. c−g Replication assays were carried out in hamster pancreatic cancer cell lines, HPD1NR, Hap-T1, SHPC6 and IPAN, and hamster kidney cancer cells (HaK) at an MOI of 5 PFU/cell. Mean and SEM are shown for each group and compared using an independent t-test. *p < 0.05, **p < 0.01, ***p < 0.001