Fig. 5.

Ad-TD-nsIL-12 is a safe and effective treatment for peritoneally disseminated PaCa. 1 × 10⁷ SHPC6 cells were seeded into the peritoneal cavity of Syrian hamsters. Four days later, 10 hamsters per group were each injected i.p with PBS or viruses. a PBS or 1 × 10⁹ PFU each virus was injected i.p on days 0, 2, and 4 and survival monitored. b PBS or varying doses of Ad-TD-IL-12 (2.5 × 10⁸ to 1 × 10⁹) were injected i.p. on days 1, 2, and 4 and survival monitored. c, d Using the same tumor model, nine hamsters per group were each injected i.p with 500 µl PBS, 1 × 10⁹ PFU Ad-TD-LUC, 5 × 10⁸ PFU Ad-TD-IL-12 or 1 × 10⁹ PFU Ad-TD-nsIL-12 on day 0. Serum was collected on days 1, 3, and 5 for detection of the levels of ALT, AST, and ASP, respectively, c or IL-12 d detected by ELISA. Mean and SEM are shown. Statistical analysis was carried out using a one-way ANOVA with post hoc Tukey’s Multiple Comparison Test or independent t-test. *p < 0.05, **p < 0.01, ***p < 0.001. e 3 × 10⁹ PFU Ad-TD-nsIL-12 or PBS was administered i.p on day 0 and survival monitored (n = 10). f PBS or 1 × 10⁹ PFU Ad-TD-nsIL-12 was injected i.p on days 0, 2 and 4 (n = 10). Forty days later, surviving hamsters previously treated with Ad-TD-nsIL-12 were re-challenged with 1 × 10⁷ SHPC6 cells. Age-matched naive hamsters also were peritoneally injected with SHPC6 cells as control. Survival was monitored. In each case, Kaplan–Meier survival curves were generated and significance assessed using the log-rank (Mantel–Cox) test. **p < 0.01, ***p < 0.001