Fig. 8.

Ad-TD-nsIL-12 efficacy is mediated by production of inflammatory mediators. 2 × 10⁶ HPD1NR cells were seeded into the right flank of Syrian hamsters. When tumor volumes reached 300 mm³, nine hamsters per group were each injected i.t with 100 µl PBS, 1 × 10⁹ PFU Ad-TD-LUC or Ad-TD- IL-12/nsIL-12 on day 0. Spleens, draining lymph nodes (DLN) and tumors were collected and analyzed by qPCR to analyze expression levels of CD83, IFN-γ, IP10, IL-1β, IL-2, IL-6, IL-10, IL-12, and IL-21 at the indicated time points. All experiments were performed in triplicate and the mean and SEM are shown for each group compared using an independent t-test. *p < 0.05, **p < 0.01, ***p < 0.001