Fig. 4.

STD and LOW flies of both sexes produce and respond to autotoxins. a, b Survival of LOW iso31 responder males is decreased more by vials preconditioned with 15 STD a than with an equivalent biomass of 30 LOW b males. Survival curves shown in b are a subset of those shown in Fig. 3f and are repeated here to facilitate side-by-side comparisons with the data in a. c Survival of STD responder males (10 per vial) in vials preconditioned with 30 LOW males. d Survival of STD responder males (15 per vial) in vials preconditioned with 30 STD males. e Survival of LOW responder females (15 per vial) in vials preconditioned with 15 STD males. f Survival of LOW responder males (15 per vial) in vials preconditioned with 30 STD males or 30 STD females. g The tendency of females to survive longer than males is reversed by housing adults at a lower density (15 per vial; left graph) or by a LOW developmental dietary history (right graph). All graphs use HYHG adult diet and show a survival curve with non-preconditioned control vials (none). To aid comparison, and as the experiments were performed in parallel, the curves with no donors (none) are the same in a, f and the curves for STD males and females (30 per vial) are also the same in both graphs in g