Fig. 8.

Autotoxin levels are sensitive to both larval and adult diet. a Larval dietary history regulates cuticular 7(Z)-tricosene levels in white Dahomey males at 3 weeks of age. LOW and STD flies were sorted into three weight bins, revealing that LOW heavy flies have significantly less 7(Z)-tricosene than STD light flies, despite being close in body size (n = 3 for LOW light and LOW medium, n = 5 for LOW heavy, n = 6 for STD light and STD medium, and n = 4 for STD heavy). b Adult diet influences cuticular 7(Z)-tricosene levels in STD iso31 males at 3 weeks of age. The levels of the four major male cuticular hydrocarbons are higher on HYHG than on LY, HY or HG adult diets (n = 6). ***p < 0.001, two-way ANOVA followed by the Bonferroni multiple comparisons test. Error bars show 1 s.d.