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. 2017 Nov 9;7:15140. doi: 10.1038/s41598-017-15474-7

Figure 3.

Figure 3

Effect of NAC on hypoxia-induced HIF1α and EGFR signalling. MDA-MB-468 cells were treated with NAC (10 mM) and exposed to hypoxia for 6 h, 24 h and 48 h. (A) Representative cropped immunoblot and (B) densitometry analysis of HIF1α protein levels (normalised to β-actin). (C) Representative cropped immunoblot and densitometry analysis of (D) total EGFR (normalised to β-actin) and phosphorylated EGFR (p-EGFR) (Y1173) levels (normalised to β-actin and total EGFR) (full-lenght immunoblots are shown in the Supplementary Fig. S2). *p < 0.01, **p < 0.001 (two-way ANOVA, with Tukey’s multiple comparisons test). All densitometry data presented are the mean of three independent experiments ± SD.