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. 2017 Nov 9;7:15140. doi: 10.1038/s41598-017-15474-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Trolox and Tempol on hypoxia-induced N-cadherin and SERPINE1 expression and EGFR activation. MDA-MB-468 cells were treated with Trolox (10 and 100 µM) and Tempol (0.1 and 1 mM) and placed under hypoxic conditions for 24 h. The mRNA levels of N-cadherin and SERPINE1 following Trolox (A) and Tempol (B) treatments were assessed using real time RT-PCR. Levels of EGFR phosphorylation (normalised to β-actin and total EGFR) following Trolox (C) and Tempol (D) treatments were assessed using immunoblotting (representative cropped immunoblots are shown in the figure; full-lenght immunoblots are shown in the Supplementary Fig. S2). ns = not significant (p > 0.05), *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA, with Tukey’s multiple comparisons test). Graphs represent the mean ± SD for three independent experiments.