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. 2017 Nov 9;7:15130. doi: 10.1038/s41598-017-15417-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The step-by-step approach used to identify new endocrine cell biomarkers. Schematic overview of the approach taken to mine for new endocrine cell biomarkers in the transcriptome of human islet preparations (n = 5) analysed by RNA sequencing¹² under both control condition and following treatment with pro-inflammatory cytokines (IL-1β + IFN-γ). Enriched pancreatic islet specific transcripts were identified by comparing transcriptomes of human pancreatic islets against 16 different normal human tissues. IPA: ingenuity pathway analysis, http://www.ingenuity.com/products/ipa.