Figure 1.

Work flow of cell robot based screening. A two-step screening strategy is suggested. First, phages are absorbed by engineered cells and packaged (scored) based on the performance of the molecules carried by the phages. Only the phages carrying molecules with desired properties are packaged in an effective way. Second, the ‘scored’ phages are absorbed by fresh host cells. In this step, only the infective phages, i.e. phages carrying molecules with the desired properties, are ‘absorbed’ by the host cells. The cells infected by the phages with desired molecules/targets acquire kanamycin resistance and can be easily identified by cultivation under kanamycin stress.