Table 3.
List of predicted proteases with calculated activity score.
| Protease | ↓pept. | ↑pept. | %freq | Score | Transcriptomics expression (Nephroseq; in DN vs. controls) | Protein expression (Musante et al.4; in DN vs. diabetics) |
|---|---|---|---|---|---|---|
| Activated | ||||||
| CTSD | 1 | 9 | 80 | 9.65 | increase | increase |
| KLK4 | 7 | 100 | 7.53 | — | — | |
| PCSK4 | 7 | 100 | 7.53 | — | — | |
| PCSK5 | 7 | 100 | 7.53 | inconclusive* | — | |
| PCSK6 | 7 | 100 | 7.53 | decrease | — | |
| PCSK7 | 7 | 100 | 7.53 | inconclusive* | — | |
| MMP-7 | 5 | 100 | 5.38 | increase | increase | |
| CTSK | 3 | 100 | 3.23 | increase | — | |
| MMP-26 | 3 | 100 | 3.23 | decrease | — | |
| MMP-20 | 2 | 3 | 20 | 1.60 | — | — |
| MMP-1 | 1 | 2 | 33 | 1.51 | increase | — |
| Deactivated | ||||||
| MMP-13 | 10 | 4 | −43 | −12.05 | decrease | no change |
| MMP-9 | 10 | 6 | −25 | −7.57 | — | decrease |
| MMP-2 | 4 | 1 | −60 | −6.36 | increase | no change |
| MMP-3 | 4 | 1 | −60 | −6.36 | increase | increase |
| MMP-8 | 5 | 2 | −43 | −6.02 | decrease | no change |
| MMP-12 | 4 | 2 | −33 | −3.89 | — | no change |
(↓ pept.: number of downregulated peptides, ↑ pept.: number of upregulated peptides). *Inconclusive: some studies reported increased and some decreased levels of the respective protease mRNA in DN (studies are listed in Supplementary Table 4).