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. 2017 Nov 9;7:15208. doi: 10.1038/s41598-017-15207-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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pAKT mainly expressed in macrophages in AAA tissues. Aortic sections from rats 28 days after PPE infusion were stained with antibody against pAKT and CD68 or CD31 respectively. (A) Antibody against pAKT was conjugated with Alexa Fluor 488 (green color) and antibody against CD68 or CD31 was conjugated with Alexa Fluor 546 (red color). Cell nucleus was stained with DAPI (blue color). Images obtained from fluorescence microscope were merged to locate the expression of pAKT in AAA tissues. (B) Positive cells of pAKT, or CD68, or DAPI or pAKT and CD68 cells in media and adventitia were counted and data present as mean and SD per HPF (200×). (C) Positive cells of pAKT, or CD31, or DAPI or pAKT and CD31 cells in media and adventitia were counted and data present as mean and SD per HPF (200×). HPF, High-power field..