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. 2017 Nov 9;7:15120. doi: 10.1038/s41598-017-15485-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of cabergoline and a dopamine D₁ receptor agonist on steroidogenic enzyme and ACTH-R expression. (a) RT-PCR analysis of steroidogenic enzyme mRNA expression. (b) StAR mRNA expression levels in OSR1⁺ cells treated with 20 μM cabergoline and 1 µM SKF 83822. Expression levels are normalised to levels of a housekeeping gene, β-actin. Student’s t-test was used for comparison of two groups. *P < 0.05. Data represent mean ± SEM of four independent experiments (n = 4). (c) RT-PCR analysis of mRNA expression of ACTH receptor in OSR1⁺ cells and SF-1-transfected OSR1⁺ IM cells.