Table 1.

Levels of steroid hormones secreted into culture medium.

	Control	ACTH	ACTH + SKF 83822
Cortisol (pg/ml)	415 ± 19.9	**560 ± 26.2	* †1136 ± 215
Aldosterone (pg/ml)	14.5 ± 0.9	19.5 ± 2.0	*27.1 ± 3.5
DHEA (pg/ml)	2139 ± 278	2291 ± 274	*3059 ± 126

Culture medium was collected for steroid hormone measurements after a 48 h of incubation with 2.4 µM ACTH and/or 1 µM SKF 83822. Statistical analysis was performed using two-tailed unpaired Student’s t-test with Holm-Bonferroni correction. *, vs Control P < 0.05; **, vs Contol P < 0.01; ^†, vs ACTH P < 0.05. Data represent mean ± SEM of five independent experiments (n = 5).