Fig. 2.

Myosin relocalizes to the cleavage furrow through cortical flow. Myosin::Dendra2 was photoconverted on the (a) apical, (b) basal and (c) lateral neuroblast cortex. Top row: maximal projection of photoconverted Myosin. Bottom row: overlay between non-photoconverted (green) and photoconverted Myosin (red), shown in maximal projection. The purple dashed box represents the photoconverted ROI. d Representative wild-type neuroblasts showing photoconverted Myosin at the apical (top) or e basal cortex (bottom). Top row: maximal projection of photoconverted Myosin. Bottom row: maximal projection showing both non-photoconverted (green) and photoconverted Myosin (red). Myosin flow velocity was determined with custom-made software and plotted in f. Center values and error bars represent the mean and standard deviation (s.d), respectively. Asterisks denote statistical significance, derived from unpaired t tests: *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Each measured cell (n) is represented with a dot in the scatter plots. For each experiment, the data were collected from at least 3 independent experiments. For each independent experiment, at least 5 larvae were dissected. g Representative wild-type neuroblast expressing Sqh::GFP (Myosin; white). The GFP signal was bleached in two regions of interests (ROIs; orange dashed boxes) and kymographs (shown below the image sequence) were used to measure the recovering fluorescence. The shown kymograph was derived from the left lateral side as shown in the cartoon. Highest fluorescence intensity is shown in red, lowest in blue. Time: seconds (s). Scale bar: 5 µm. Time scale bar (open black box) in (g): 1 s. n.s. not significant