Fig. 6.

The anaphase spindle is necessary for focusing Myosin localization at the cleavage furrow. a Laser-induced cortical cutting experiments performed on third instar wild-type neuroblasts expressing Sqh::GFP (Myosin, white; upper panel, green; lower panel) and Cherry::Jupiter (MTs; magenta) in metaphase, or b anaphase. For this and subsequent panels, the cortical cutting site is highlighted with purple lines. Orange arrowheads highlight Myosin recruitment. Myosin intensity profiles, measured from the apical to the basal cortex, are shown underneath the corresponding image. c Laser-induced cortical cutting experiments performed on third instar rod mutant neuroblasts exposed to colcemid in anaphase. Only Sqh::GFP (Myosin; white) is shown. d Experimental summary. Time is shown as seconds before and after cortical cutting. Scale bars: 5 µm