Fig. 3.

Increased ZOL-sensitive tonic current in DMV neurons from STZ-treated mice. A: representative traces of ZOL-induced (1 mM) tonic currents in saline (left)- and STZ-treated mice (right). Dashed lines represent baseline holding current. aCSF contained GABA (3 μM) and kynurenic acid (1 mM). B: group statistics for ZOL-induced I-tonic current density for all neurons in saline (n = 8)- and STZ-treated mice (n = 9) with overlaid individual neuronal responses. C: group statistics for ZOL-induced total tonic current density for both groups, with overlaid individual neuronal responses. D: pie chart illustrating the significant increase in the proportion of cells expressing significant ZOL-induced responses. *P < 0.05 (Mann-Whitney U-test). E: correlation between ZOL-induced I-tonic current density as a function of sIPSC decay time constant. Because short sIPSC decay times did not predict the amplitude of ZOL-induced tonic current density (P > 0.05), hyperglycemia/hypoinsulinemia-mediated plasticity of ZOL-sensitive inhibitory synaptic and tonic currents in the DMV are likely to affect receptors at the synapse and those at peri-/extrasynaptic locations independently.