Figure 2.

Messenger RNA and protein expression and enzymatic activity of ALDH isozymes in human CRC cell lines. (A) Messenger RNA expression levels were measured by real-time PCR. Data are presented as the mean of three replicates. (B) Western blot analysis of ALDH1A1, ALDH1B1, ALDH2, and β-actin. Ten μg of protein was applied to each lane. (C) ALDH enzymatic activity. Substrates tested include acetaldehyde, proprionaldehyde, and benzaldehyde. Data are presented as the mean and associated s.e.m. (n=3).