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. 2017 Sep 14;117(10):1495–1506. doi: 10.1038/bjc.2017.317

Figure 4.

Figure 4

Camptothecin (CPT) sensitises HepG2 cells to As2O3-induced cytotoxicity. Veh=Vehicle (medium). (A) The HepG2 cells were exposed to various concentrations of As2O3 in the presence or absence of 0.1 μM CPT for 24 h and subsequently the cell viability was measured by MTS assay; n=6. *P<0.05 vs Control with the same As2O3 treatment. (B) CPT enhances the cytotoxicity of As2O3 in a concentration-dependent manner. The HepG2 cells were treated with 10 μM As2O3 in the presence of different concentrations of CPT; n=6. *P<0.05 vs Control with the same As2O3 treatment; #P<0.05 vs Control with Veh. (C) The HepG2 cells were treated with 10 μM As2O3 in the presence or absence of 0.1 μM CPT for 24 on a xCELLigence real-time cell analysis system to detect cell growth and proliferation. The proliferation curves (left panel) and cumulative data (right panel) of cells at different times; n=3. *P<0.05 vs Control at the same time. #P<0.05 vs As2O3 at the same time. (D) Cytotoxicity of As2O3 in the Scramble and NFE2L2-KD cells. Cells were treated with various concentrations of As2O3 in the presence or absence of 0.5 μM CPT in Scramble or NFE2L2-KD cells for 24 h; n=6. *P<0.05 vs Control with the same As2O3 treatment. A full colour version of this figure is available at the British Journal of Cancer journal online.