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. 2017 Oct 2;3:17067. doi: 10.1038/cddiscovery.2017.67

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression studies of TLR4 signaling pathway-related genes. Spleen was removed from control and infected zebrafish at indicated time points, total RNA isolated, cDNA prepared which was used for qPCR analysis of (a) TLR4; (b) MyD88, TRIF; (c) IRAK4, RIP1, TRAF6, NFκB; (d) TNF-α, IL-1β, IL-4, IL-10. Each bar represent the mean of three independent experiments (n=5/experiment) and the error bars represent the standard deviations. C, control; h, hours; d, days; and asterisks '∗' on bars indicate significant difference from control (P<0.05).