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. 2017 Oct 2;3:17067. doi: 10.1038/cddiscovery.2017.67

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Copyright © 2017 The Author(s)

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Subcellular distribution of TLR4. (a) zSPM were isolated from control and infected fish, stained with Hoechst 33342, TLR4 and EEA1 antibody for TLR4 internalization study (×100). (b) Expression of p110δ was determined from spleen of control and infected fishes. Each bar represent the mean of three independent experiments (n=5/experiment) and the error bars represent the standard deviations. C, control; d, days; h, hours; zSPM, zebrafish splenic macrophages and asterisks '∗' on bars indicate significant difference from control (P<0.05).