Table 1. List of real-time primers used for indicated genes of zebrafish.
| Gene | Forward primer | Reverse primer | Accession number | Product length (bp) |
|---|---|---|---|---|
| β Actin (actb1) | CGAGCAGGAGATGGGAACC | CAACGGAAACGCTCATTGC | FJ915059.1 | 104 |
| TLR4a | CGGCACTCCTCAAATCAACT | GTCCTTCAAATCCTCCCACA | NM_001131051 | 77 |
| TLR9 | CACGGACACCCAGTATGATG | GGAATCCAGTCACGCTCTTC | NM_001130594 | 147 |
| MyD88 | AGTTTGCGCTCAGTCTTTGC | ACAGATGGTCAGAAAGCGCA | NM_212814.2 | 110 |
| TRIF (ticam1) | GCCGAAGAGTCGTAGACCTG | CCTCTCCCAAATTCCGTCCC | NM_001044759.1 | 167 |
| IRAK4 | TACTGGACGAGGGTTTTGTGG | CGCACTCGAGCTATCCTTCATC | BC164777.1 | 153 |
| RIP1 (ripk1l) | TCCTGGACCAAACCATCAGC | ACTGACTCCTCAATTCGGGC | NM_001043350.1 | 100 |
| TRAF6 | ACTAGAGGAGAGCACCCGAG | GGAGGACAATAGGCTGACCG | NM_001044752.1 | 119 |
| TRAF3 | GCTGTGGTCTCCAAAACCCT | GAGCGAGAGATGTGTGCCTT | NM_001003513.1 | 126 |
| NFκB (relA) | AAAAGATGGAGCCCTCACCC | ATCAGCCTTGCATCCCTACC | NM_001001839.2 | 148 |
| TNF-α | GCGCTTTTCTGAATCCTACG | TGCCCAGTCTGTCTCCTTCT | AY427649 | 149 |
| IL-1β | TGGACTTCGCAGCACAAAATG | CGTTCACTTCACGCTCTTGGATG | AY340959 | 151 |
| IFN-γ | ATGATTGCGCAACACATGAT | ATCTTTCAGGATTCGCAGGA | AB158361 | 189 |
| IL-12 | AGCAGGACTTGTTTGCTGGT | TCCACTGCGCTGAAGTTAGA | AB183001 | 145 |
| IL-4 | CATCCAGAGTGTGAATGGGA | TTCCAGTCCCGGTATATGCT | AM403245.2 | 200 |
| IL-10 | ATTTGTGGAGGGCTTTCCTT | AGAGCTGTTGGCAGAATGGT | NM_001020785 | 199 |
| P110δ (pik3cd) | TCTCTGGTCACTCGAACCGA | GTGCTTTCTCCTGACCCGAA | NM_201199.1 | 100 |
| RAB7 | ATCACTGGCCTTTGTAGACGAG | GGCGATTTTGCAGAAGTGGTG | NM_200928.1 | 155 |
| NOD2 | GAGTTCTGTTGTGACTGGGCT | TCGCTACCTCCACCACATAGA | NM_001328044.1 | 114 |