Figure 5.

The interaction between Cathepsin S, LC3 and Ubiquitin. (a) HeLa cells were pre-treated with CQ for 1 h and co-treated with hispolon for 23 h. Co-stained with LC3 and CTSS antibody and observed by confocal microscopy. (b) HeLa and SiHa cells were pre-treated with CQ for 1 h and co-treated with hispolon for 23 h then analyzed by western blot. (c) After treating the cells with hispolon, detecting that LC3 interacted with CTSS by Co-IP. (d) The cells were treated with hispolon and co-treated with CQ then detecting the interaction between Cathepsin S and Ubiquitin by CO-IP