Figure 3.

Pro-oxidant alone or its priming has little effect on mitophagy. (a) HeLa cells stably expressing HA-Parkin treated with DMSO, H₂O₂ (100 μM), antimycin A (2 μM) or CCCP (10 μM) for 2 h. Cells were stained for hsp60 (red) and HA (purple). (b) HeLa cells stably expressing HA-Parkin treated with DMSO, H₂O₂ (100 μM), antimycin A (2 μM) or CCCP (10 μM) for 24 h. Cells were stained for HA (green) and hsp60 (red). GFP-Parkin-overexpressing cells were treated with DMSO or antimycin A for 2 h prior to CCCP treatment for 4 h (c) or 24 h (d). Cells were stained with hsp60 (white). Distribution of Parkin and reduction in mitochondrial mass were quantified on the right. White arrowhead indicates cytosolic Parkin; yellow arrows indicate punctate-shaped Parkin; yellow arrowheads indicate Parkin aggregates. NS, not significant