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. 2017 Oct 12;8(10):e3098. doi: 10.1038/cddis.2017.469

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Expression of BCL-2 family genes is altered by CAV1 in HCC. HLE shControl, HLE shCAV1, Huh7 +pControl and Huh7 +pCAV1 were treated with TGF-β (2 ng/ml) at the times shown after previous FBS starvation (2% FBS; 4 h). (a and b) Relative expression levels of BMF and BIM; L32 was used as house-keeping gene (N=2). Results are expressed as mean±S.E.M. (c) Upper panel: immunoblot of total protein extracts; β-ACTIN is presented as loading control. A representative experiment is shown (N=3). Lower panel: densitometric analysis of BIM relative levels; results are mean±S.E.M. of three independent experiments and are expressed as absolute values. Statistical comparison was done by two-way ANOVA with Sidak post-hoc test as shown in the figure: *P<0.05, **P<0.01, ***P<0.001